That it phospho-threonine acts as a beneficial rigidifying centre, stabilizing the new activated kind of the newest kinase heterodimer [18, 19]
Upon binding of your cyclin to help you Cdk2, brand new CDK C-helix packages up against one to particular helix regarding the cyclin companion compliment of a surface characterized by comprehensive hydrophobic interactions. Organization off cyclins on the C-helix produces a rotation regarding axis from the sector, producing brand new relationships which might be the main productive ATP-joining website. As well, cyclins make the C-lobe activation sector out from the catalytic website and so the threonine gets available getting activating phosphorylation by CAK (Profile 3).
